We attend prenatal diagnosis of trisomy mosaic of double trisomy 11 and trisomy 12 in amniocentesis with a single colony in 23 years outcome.A benefit women undergoing amniocentesis at 24 weeks of gestation because of congenital fetal bowel dilation. Amniocentesis revealed a karyotype 48, XX, + 11, + 12 [1] / 46, XX [24]. In the 25 colonies cultured amniocytes, all five cells in one colony had karyotype 48, XX, + 11, + 12, while the remaining 24 colonies had karyotype 46, XX. The karyotype of normal parents. Repeat amniocentesis perform ed at 26 weeks gestation. interphase fluorescence in situ hybridization (FISH), array comparative GENTAUR genomic hybridization (aCGH) and fluorescent quantitative polymerase chain reaction (QF-PCR) is applied to the uncultured amniocytes, and conventional cytogenetic analysis applied to cultured amniocytes. Interphase FISH analysis showed no signal of trisomy 11 and trisomy 12 was no signal in 102 uncultured amniocytes. QF-PCR analy...